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叶片和生殖器官的调节对Bt棉杀虫蛋白特性的影响

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叶片和生殖器官的调节对Bt棉杀虫蛋白特性的影响
论文目录
 
Abstract第7-11页
中文摘要第11-13页
Charter 1. General introduction第13-35页
  1. Overview for transgenic cotton第13页
  2. Bt cotton growth and development第13-14页
  2.1 Introduction of Bt gene on vegetative and reproductive organs第13-14页
  2.2 Introduction of Bt gene on yield and components第14页
  3. Overview for insect resistance in Bt cotton第14-18页
  3.1 Bt transgenic cotton can kill bollworm significantly第14-15页
  3.2 Temporal and Spatial Variation in Insecticidal Efficacy in Bt Cotton第15-17页
  3.3 The pest could evolve resistance to Bt cotton第17-18页
  4. Factors affected expression of insect resistance in Bt cotton第18-22页
  4.1 Effects of Environmental Stress on insecticidal efficacy in Bt cotton第18-20页
  4.2 Mechanisms of Changes in insecticidal efficacy in Bt cotton第20-22页
  5. Nitrogen application on growth and insect resistance in Bt cotton第22-23页
  5.1 nitrogen on growth第22-23页
  5.2 nitrogen on insect resistance第23页
  6. Research objectives第23-25页
References第25-35页
Charter 2. Changed Growth Characteristics with Bacillus thuringiensis GeneIntroduction and Nitrogen Regulation in Bt Cotton第35-55页
  1. Introduction第36-37页
2 Materials and methods第37-39页
  2.1 Plant Material and Experimental Design第37-38页
  2.2 Measurements第38页
  2.3 Statistical Analysis第38-39页
3 Results第39-49页
  3.1 Effect of the Introduction of the Bt Gene into Cotton on Vegetative Organ Growth第39-41页
  3.2 Effect of Introducing the Bt Gene into Cotton on Reproductive Organ Development第41-45页
  3.3 Effect of Nitrogen Regulation on Vegetative and Reproductive Organ Growth andDevelopment第45-49页
4 Discussion第49-51页
  4.1 Effect of the Introduction of the Bt Gene on Vegetative Growth and ReproductiveDevelopment in Cotton第49-50页
  4.2 Effect of Nitrogen Application on Vegetative and Reproductive Growth of Bt Cotton第50-51页
5 Conclusion第51-52页
References第52-55页
Charter 3. Amino acid Composition and Level affect Bt Protein Concentration in BtCotton第55-75页
  1. Introduction第56-57页
  2. Materials and methods第57-60页
    2.1. Plant material and experimental design第57-58页
  2.2 Preparation of samples第58-59页
  2.3 The CrylAc protein concentration assay第59-60页
  2.4 Determination of amino acids第60页
  2.5 Statistical analysis第60页
  3. Results第60-67页
  3.1 Effect of the temperature stress on leaf Bt protein and amino composition in Bt cotton第60-64页
  3.2 The effect of inhibitors application of GPT and GS on leaf Bt protein and amino acidcomposition in Bt cotton第64-67页
  4. Discussion第67-70页
  4.1 The change of amino acid metabolism by external influence related to developmentstage in Bt cotton第67-69页
  4.2 The differences in amino acid composition and their level affected Bt protein concentration inBt cotton第69-70页
  5. Conclusions第70页
References第70-75页
Charter 4. Square Size affect Bt Protein Property in Bt Cotton第75-115页
  1. Introduction第76-77页
  2. Materials and methods第77-83页
    2.1. Plant materials and field design第77-79页
    2.2. Preparation of plant material第79页
    2.3. Physiological measurements第79-82页
      2.3.1.Square volume and dry weight第79页
      2.3.2. The CrylA protein concentration assay第79-80页
      2.3.3. Assay of free amino acid and soluble protein content第80-81页
      2.3.4. Glutamic-pyruvic transaminase(GPT) and Glutamate-oxaloacetate transaminase (GOT)assay第81页
      2.3.5. Assay of protease and peptidase activity第81-82页
      2.3.6. Total nitrogen content assay第82页
    2.4. Statistics analysis第82-83页
  3. Results第83-108页
  3.1 square size on insecticidal property for different type of cultivars第83-85页
    3.1.1 Difference of Square size between Bt cultivars第83-84页
    3.1.2 CrylAc insecticidal protein contents of Square between cultivars第84-85页
  3.2 Square size on insecticidal property under different planting density for the cultivars第85-91页
    3.2.1 Square size under different planting density第85-86页
    3.2.2 Square Bt toxin content under different planting density第86-88页
    3.2.3 relationship between square size and Bt insecticidal content under different plantingdensity第88页
    3.2.4 Square nitrogen metabolism under different planting density第88-91页
      3.2.4.1 Amino acid and soluble protein content第88-89页
      3.2.4.2 GPT and GOT第89-90页
      3.2.4.3 protease and peptidase第90-91页
  3.3 Square size on insecticidal property under different nitrogen rates for the cultivars第91-97页
    3.3.1 Square size under different nitrogen rates第91-92页
    3.3.2 Square Bt toxin content under different nitrogen rates第92-93页
    3.3.3 relationship between square size and Bt insecticidal content under different nitrogenrates第93-94页
    3.3.4 Square nitrogen metabolism under different planting density第94-97页
      3.3.4.1 Amino acid and soluble protein content第94-95页
      3.3.4.2 GPT and GOT第95-96页
      3.3.4.3 Protease and peptidase第96-97页
    3.4. Square size on insecticidal property by leaf-square regulation for the cultivars第97-108页
    3.4.1 The effect of LC and SR on square size第97-99页
    3.4.2 The effect of SR and LC on Square CrylAc insecticidal protein content第99-100页
    3.4.3 The relationship between Square size and insecticidal protein content under artificialtreatments第100-102页
      3.4.4. The nitrogen metabolism characteristics under artificial treatments of LC and SR第102-108页
  4. Discussion第108-111页
    4.1. Square size affected Bt toxin concentration第108-109页
    4.2. Big square formation in breeding and cultural practices benefit to increase insect resistance第109-111页
References第111-115页
Charter 5. Boll Number and Size on Boll Wall Insecticidal Protein Content in BtCotton第115-155页
  1. Introduction第116-117页
  2. Materials and Methods第117-122页
    2.1. Plant materials and field design第117-118页
    2.2. Preparation of plant material第118-119页
    2.3. Physiological measurements第119-121页
      2.3.1. Boll volume第119页
      2.3.2.The CrylAc protein concentration assay第119-120页
    2.3.3 Assay of free amino acid and soluble protein content第120页
      2.3.4. Glutamic-pyruvic transaminase (GPT) and Glutamate oxaloacetatetransaminase (GOT)assay第120-121页
    2.3.5 Assay of protease and peptidase activity第121页
    2.4. Statistics analysis第121-122页
  3. Results第122-149页
  3.1 boll number and size on insecticidal property under different planting density for the cultivars第122-132页
    3.1.1 Boll number and size under different planting density第122-124页
    3.1.2 boll wall Bt toxin content under different planting density第124-125页
    3.1.3 relationship between boll number, size and boll wall Bt insecticidal content underdifferent planting density第125-127页
    3.1.4 boll wall nitrogen metabolism under different planting density第127-132页
      3.1.4.1 Amino acid and soluble protein content第127-128页
      3.1.4.2 GPT and GOT第128-130页
      3.1.4.3 protease and peptidase第130-132页
  3.2 boll number and size on insecticidal property under different nitrogen rates for the cultivars第132-140页
    3.2.1 boll number and size under different nitrogen rates第132-134页
    3.2.2 boll wall Bt toxin content under different nitrogen rates第134-136页
    3.2.3 relationship between boll number, size and boll wall Bt insecticidal content underdifferent nitrogen rates第136-137页
    3.2.4 boll wall nitrogen metabolism under different nitrogen rates第137-140页
      3.2.4.1 Amino acid and soluble protein content第137-138页
      3.2.4.2 GPT and GOT第138-139页
      3.2.4.3 protease and peptidase第139-140页
    3.3. boll number, size on boll wall insecticidal property by leaf-square regulation for the cultivars第140-149页
    3.3.1 The effect of LC and SR on boll size第140-141页
    3.3.2 The effect of LC and SR on boll wall Bt protein content第141-142页
    3.3.3 relationship between boll size and boll wall Bt insecticidal content under LC and SR第142-143页
    3.3.4 boll wall nitrogen metabolism under LC and SR第143-149页
      3.3.4.1 Amino acid and soluble protein content第143-145页
      3.3.4.2 GPT and GOT第145-147页
      3.3.4.3 protease and peptidase第147-149页
  4. Discussions第149-151页
  5. Conclusion第151页
References第151-155页
Acknowledgement第155-156页
The publications of the author第156-158页

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