论文目录 | |
| Acknowledgement | 第1-16页 |
| Abstract | 第16-18页 |
| 摘要 | 第19-20页 |
| Introduction | 第20-22页 |
| Background information for the experimental materials | 第22-23页 |
| Objectives of the study | 第23-24页 |
| Chapter1 Review of literature | 第24-40页 |
| 1.1 Regulations of leaf senescence | 第24-30页 |
| 1.1.1 Senescence process | 第24-25页 |
| 1.1.2 Structural and biochemical changes in leaf during senescence | 第25-27页 |
| 1.1.3 Involvement of phytohormone in the regulation of leaf senescence | 第27-30页 |
| 1.2 Effect of Nitrogen supply on leaf senescence | 第30-35页 |
| 1.2.1 Photosynthetic N metabolism and assimilation | 第31-32页 |
| 1.2.2 Photosynthetic carbon(C)metabolism during senescence | 第32-35页 |
| 1.2.3 Regulation of C and N metabolism in response to nitrogen availability | 第35页 |
| 1.3 Effect of N on nonstructural carbohydrate(NSC)reserve and remobilization | 第35-37页 |
| 1.4 Molecular approaches to understanding leaf senescence | 第37-40页 |
| Chapter2 Relationship of Nitrogen Deficiency-Induced Leaf Senescence with ROS Generation and ABA Concentration in Rice Flag Leaves | 第40-63页 |
| 2.1 Introduction | 第40-43页 |
| 2.2 Materials and Methods | 第43-48页 |
| 2.2.1 Plant materials and nitrogen treatment | 第43页 |
| 2.2.2 Exogenous ABA incubation experiment in detached leaves | 第43-44页 |
| 2.2.3 Measurement of net photosynthetic rate(Pn),chlorophyll content,hydrogen peroxide(H2O2)concentration,malondialdehyde(MDA)level,soluble protein content,and ABA concentration in rice leaves | 第44-45页 |
| 2.2.4 Enzymatic assay of glutamine synthetase(GS)and glutamate dehydrogenase(GDH)in rice leaves | 第45-46页 |
| 2.2.5 RNA extraction,cDNA preparation,and Quantitative real-time PCR | 第46页 |
| 2.2.6 Statistical analysis | 第46页 |
| 2.2.7 Design of key enzyme primers for regulating ABA synthesis and catabolism | 第46-48页 |
| 2.3 Results | 第48-58页 |
| 2.3.1 Senescence-associated changes in endogenous ABA accumulation,H2O2 production,and activity of key enzymes involving in nitrogen metabolism under different N supply levels | 第48-51页 |
| 2.3.2 Transcriptional expression of some key genes involving in ABA biosynthesis and its catabolism for two rice genotypes imposed to different N levels | 第51页 |
| 2.3.3 Linking the exogenous ABA-regulated leaf senescence with H2O2 generation and N metabolism in rice leaves | 第51-58页 |
| 2.4 Discussion | 第58-62页 |
| Conclusion | 第62-63页 |
| Chapter3 Senescence-Related Translocation of Nonstructural Carbohydrate(NSC)in Rice Leaf Sheathes Under Different Nitrogen Supply | 第63-85页 |
| 3.1 Introduction | 第63-65页 |
| 3.2 Materials and Methods | 第65-68页 |
| 3.2.1 Plant materials and nitrogen treatment | 第65-66页 |
| 3.2.2 Measurement of net photosynthetic rate(Pn)and chlorophyll content in rice leaves | 第66页 |
| 3.2.3 Determination of soluble sugar content, starch content, and NSC amount in leaf sheaths | 第66-67页 |
| 3.2.4 Enzymatic extraction and assays of SPS,FBPase,Susy,GS,GOT,and GPT in leaf sheaths | 第67-68页 |
| 3.2.5 Statistical analysis | 第68页 |
| 3.3 Result | 第68-76页 |
| 3.3.1 Effect of N supply levels on leaf senescence and grain yield and also its relation to the NSC translocation and starch accumulation in leaf sheaths | 第68-69页 |
| 3.3.2 Positional variations in the NSC amount,total soluble sugar content,and starch accumulation in leaf sheathes under different N supply levels | 第69-75页 |
| 3.3.3 Effect of N supply levels on the activities of several key enzymes involving in NSC metabolism in the leaf sheath and its positional variation among three leaf sheaths | 第75-76页 |
| 3.4 Discussion | 第76-84页 |
| Conclusion | 第84-85页 |
| Chapter4 Senescence-Related Changes in Nitrogen Metabolism and Carbohydrate translocation in Rice Leaves under Different Nitrogen Supply | 第85-113页 |
| 4.1 Introduction | 第85-87页 |
| 4.2 Materials and Methods | 第87-92页 |
| 4.2.1 Plant Materials and Nitrogen Treatment | 第87-88页 |
| 4.2.2 Determination of net photosynthetic rate(Pn),chlorophyll content,and Relative conductivity | 第88页 |
| 4.2.4 Determination Carbon and Nitrogen metabolism-related indicators | 第88页 |
| 4.2.5 Carbon and Nitrogen Metabolism-related Enzyme Assay | 第88-89页 |
| 4.2.6 RNA isolation,cDNA preparation,and Quantitative real-time PCR | 第89页 |
| 4.2.7 Statistical analysis | 第89-92页 |
| 4.3 Results | 第92-107页 |
| 4.3.1 Senescence-associated changes in leaf Chla/Chlb,total soluble sugar,sucrose,starch,soluble protein,and total N contents under different nitrogen supply | 第92页 |
| 4.3.2 N deficiency induced C/N imbalance in the flag leaves during grain filling | 第92-99页 |
| 4.3.3 Transcriptional profile of key genes involving in carbon and nitrogen metabolism for two rice genotypes imposed to N deficiency/overfeeding | 第99-107页 |
| 4.4 Discussion | 第107-113页 |
| Chapter5 | 第113-116页 |
| 5.1 Major findings | 第113-114页 |
| 5.2 Future perspectives | 第114-116页 |
| References | 第116-141页 |
| List of Publications | 第141页 |
