论文目录 | |
| 中文摘要 | 第1-5页 |
| English abstract | 第5-9页 |
| List of abbreviations and acronyms | 第9-10页 |
| 1 PARTⅠ:REVIEW | 第10-13页 |
| 1.1 Riceproduction | 第10页 |
| 1.2 Plant growth and development | 第10-13页 |
| 1.2.1 Vegetative growth stage | 第11页 |
| 1.2.2 Leaf development | 第11-13页 |
| 2 PARTⅡ:CHARACTERIZATION AND FINE MAPPING OF A DWARF AND NARROWLEAF MUTANT GENE DNL2 IN RICE | 第13-25页 |
| 2.1 Introduction | 第13-15页 |
| 2.2 Materials and methods | 第15-16页 |
| 2.2.1 Plant materials and field experimentation | 第15页 |
| 2.2.2 Paraffin sectioning and histological analysis | 第15页 |
| 2.2.3 Gibberellic acid 3(GA3) and brassinolide(BL)induction of shoot elongation | 第15页 |
| 2.2.4 Determination of crude fiber content | 第15-16页 |
| 2.2.5 RNA extraction and quantitative real time RT-qPCR | 第16页 |
| 2.2.6 Fine mapping | 第16页 |
| 2.3 Results | 第16-23页 |
| 2.3.1 The dnl2 mutation affects plant height,plant architecture and grain yield | 第16-18页 |
| 2.3.2 Physical and biochemical characterization of the mutant's leaf and stem | 第18-20页 |
| 2.3.3 The mutant's leaf and stem were brittle | 第20-21页 |
| 2.3.4 Fine mapping and candidate genes analysis of dnl2 mutant genes | 第21-23页 |
| 2.4 Discussion | 第23-25页 |
| 3 PARTⅢ:FURTHER INSIGHT INTO THE ROLE OF ABAXIALIZED-1,AN ALLELICMUTANT OF KANADI FAMILY MEMBER KAN1 IN RICE | 第25-46页 |
| 3.1 Introduction | 第25-27页 |
| 3.2 Materials and Methods | 第27-30页 |
| 3.2.1 Plant materials and field experiments | 第27页 |
| 3.2.2 Paraffin sectioning and histological analysis of leaves | 第27页 |
| 3.2.3 Scanning electron microscopic observation of plant culms | 第27页 |
| 3.2.4 Exogenous application NAA,IAA),and GA3 | 第27-28页 |
| 3.2.5 Hormone content | 第28页 |
| 3.2.6 RNA extraction and real time quantitative PCR(qPCR) | 第28页 |
| 3.2.7 Fine mapping | 第28页 |
| 3.2.8 Plasmid construction | 第28-29页 |
| 3.2.9 Plant transformation | 第29页 |
| 3.2.10 Phylogenetic and protein structure analysis | 第29页 |
| 3.2.11 Yeast two-hybrid screening analysis | 第29-30页 |
| 3.3 Results | 第30-43页 |
| 3.3.1 The ab-1 showed several morphological defects | 第30-31页 |
| 3.3.2 The ab-1 has an altered bulliform cells in adaxial side of the leaves | 第31-32页 |
| 3.3.3 The KANADI1 transcription factor is candidate gene of ab-1 | 第32-33页 |
| 3.3.4 The AB-1 and ARF3 displayed an overlapping expression pattern | 第33-34页 |
| 3.3.5 Protein structure and phylogenetic analysis of AB-1 | 第34页 |
| 3.3.6 The AB-1 and its homologs involved in leaf polarity determination in rice | 第34-39页 |
| 3.3.7 The AB-1 might physically interact with ARFs proteins in rice | 第39-40页 |
| 3.3.8 The AB-1 is involved in IAA and GA homoeostasis in rice | 第40-42页 |
| 3.3.9 The ab-1 affected expression of putative genes involved in leaf development | 第42-43页 |
| 3.4 Discussion | 第43-45页 |
| 3.5 General conclusion | 第45-46页 |
| Acknowledgement | 第46页 |
| References | 第46-53页 |
| Supplemental data | 第53-59页 |
| 个人简历 | 第59页 |
| 博士期间发表的论文 | 第59页 |
| 博士后期间发表的论文 | 第59-60页 |
| 永久通信地址 | 第60页 |
