论文目录 | |
| ABSTRACT | 第1-8页 |
| 摘要 | 第8-12页 |
| Chapter 1 Review of Literature | 第12-37页 |
| 1.1 Research progress on powdery mildew of wheat | 第12-20页 |
| 1.1.1 Features and causes of wheat powdery mildew | 第13-15页 |
| 1.1.2 Prevention and control of the disease | 第15-16页 |
| 1.1.3 Wheat powdery mildew resistant genes and genetic researches | 第16-20页 |
| 1.2 Proteomics and recent advances in proteomic techniques | 第20-22页 |
| 1.3 Research progress on wheat proteomics | 第22-24页 |
| 1.3.1 Proteomics for studying genetic variability of wheat | 第22-23页 |
| 1.3.2 Proteomic research for biotic and abiotic stresses of wheat | 第23-24页 |
| 1.4 Use of proteomics in plant pathology and resistant breeding | 第24-33页 |
| 1.4.1 Proteomics of plant pathogenic fungi | 第26-28页 |
| 1.4.2 Proteomic research for disease resistance of rice | 第28-29页 |
| 1.4.3 Proteomic research for disease resistance of maize | 第29-31页 |
| 1.4.4 Proteomic research for disease resistance of wheat | 第31-33页 |
| 1.5 Prospects of proteomics | 第33-34页 |
| 1.6 Layout of the study | 第34-37页 |
| 1.6.1 Purpose and significance of the research | 第34-36页 |
| 1.6.2 Technical workflow of the study | 第36-37页 |
| Chapter 2 Materials and Methods | 第37-57页 |
| 2.1 Materials | 第37-43页 |
| 2.1.1 Plant material | 第37页 |
| 2.1.2 Biochemical reagents | 第37-38页 |
| 2.1.3 Major instruments and equipements | 第38页 |
| 2.1.4 Sample preparation | 第38-43页 |
| 2.2 Methods | 第43-57页 |
| 2.2.1 Seedling growth and powdery mildew inoculation | 第43页 |
| 2.2.2 Protein extraction | 第43-44页 |
| 2.2.3 Quantification of protein in the sample | 第44页 |
| 2.2.4 Two-dimensional gel electrophoresis (2-DE) | 第44-48页 |
| 2.2.5 Gel staining | 第48-49页 |
| 2.2.6 Analysis of gels for differentially expressed protein | 第49页 |
| 2.2.7 Mass spectrometry analysis | 第49-50页 |
| 2.2.8 Searching databases and protein identification | 第50-51页 |
| 2.2.9 Functional analysis | 第51页 |
| 2.2.10 Validation of selected DEPs through real-time q RT-PCR | 第51-57页 |
| Chapter 3 Results and Analyses | 第57-75页 |
| 3.1 Optimization of methods and technology | 第57-58页 |
| 3.2 Analysis of 2-DE gels for differential expression of proteins | 第58-60页 |
| 3.3 Bioinformatic analysis and identification of DEPs | 第60-61页 |
| 3.4 Functional category of identified proteins | 第61页 |
| 3.5 Expression patterns of the identified proteins | 第61-69页 |
| 3.6 Post-translational modifications of proteins | 第69-72页 |
| 3.7 Transcription analysis of the selected genes | 第72-75页 |
| Chapter 4 Discussions | 第75-93页 |
| 4.1 Wheat-Bgt interaction in relation to gene-for-gene concept | 第75-76页 |
| 4.2 Functional implications of identified proteins in wheat-Bgt interaction | 第76-90页 |
| 4.3 Proposed defensive pathway of wheat in response to Bgt infection | 第90-91页 |
| 4.4 Validation of selected genes at transcriptional level through q RT-PCR | 第91-93页 |
| Chapter 5 Summery and Innovation | 第93-96页 |
| 5.1 Summery | 第93-94页 |
| 5.2 Innovation | 第94-95页 |
| 5.3 Research prospects | 第95-96页 |
| References | 第96-117页 |
| Acknowledgements | 第117-118页 |
| About the Author | 第118页 |
