论文目录 | |
| 中文摘要 | 第1-7页 |
| Abstract | 第7-9页 |
| LIST OF ABBREVIATIONS | 第9-11页 |
| TABLE OF CONTENTS | 第11-14页 |
| Chapter 1. BACKGROUND AND LITERATURE REVIEW | 第14-35页 |
| 1.1 About health benefits | 第15-17页 |
| 1.2 Biosynthetic pathways involved in soy isoflavones | 第17-20页 |
| 1.3 MYB Transcription factor and relationship with soy isoflavone | 第20-25页 |
| 1.3.1 Types and distribution of soy isoflavones | 第22页 |
| 1.3.2 Key enzymes involved in the biosynthesis of isoflavones | 第22-24页 |
| 1.3.3 Isoflavones physiological functions | 第24-25页 |
| 1.4 Overview of GM crops and transgenic soybeans | 第25-33页 |
| 1.5 The purpose and significance of the study | 第33-35页 |
| Chapter 2. FACTORS RESPONSIBLE FOR THE EFFICIENCY OF SOYBEAN USING AGROBACTERIUM- MEDIATED TRANSFORMATION METHOD | 第35-46页 |
| 2.1 Materials | 第35页 |
| 2.1.1 Plant materials | 第35页 |
| 2.1.2 Reagents, experimental equipments and instruments | 第35页 |
| 2.2 Methods | 第35-40页 |
| 2.2.1 Effect of Optical Density (OD) services Agrobacterium on the gene transformation | 第37页 |
| 2.2.2 Effect of time of infection by means of vacuum on the gene transformation | 第37-38页 |
| 2.2.3 Effect of concentrated Acetosyringon (AS) on the gene transformation | 第38页 |
| 2.2.4 Effect of L-Cysteine concentration on the gene transformation | 第38页 |
| 2.2.5 Temporary expression of GUS | 第38-40页 |
| 2.3 Results and Discussion | 第40-45页 |
| 2.3.1 Effect of Agrobacterium tumefaciens suspention optical density (OD) on the gene transformation | 第40-41页 |
| 2.3.2 Effect of time of effection by means of vacuum on the gene transformation | 第41-42页 |
| 2.3.3 Effect of AS concentration on a temporary rate of gene expression associated GUS MYB12A. gene | 第42-44页 |
| 2.3.4 Effect of L-Cys concentration on transformation efficiency | 第44-45页 |
| 2.4 Conclusion | 第45-46页 |
| Chapter 3. THE RESEARCH ON GmMYB12A, AND GmMYB12B2 GENE TO TRANSFER EFFICIENCIES OF SOME SOYBEAN GENOTYPE BY HALF-SEED EXPLANT METHOD | 第46-87页 |
| 3.1 Materials | 第46-48页 |
| 3.1.1 Plant materials | 第46页 |
| 3.1.2 Genetic material | 第46-48页 |
| 3.1.3 Reagents, experimental equipments and instruments | 第48页 |
| 3.2 Methods | 第48-69页 |
| 3.2.1 Cloning and sequence analysis in transcriptional factor of soybean MYB12A and MYB12B2 gene method | 第49-54页 |
| 3.2.2 Agrobacterium tumefaciens preparation | 第54-55页 |
| 3.2.3 Media composition and preparation of cultures | 第55页 |
| 3.2.4 Molecular analysis | 第55-69页 |
| 3.3 Results and Discussion | 第69-86页 |
| 3.3.1 Infection and co-cultivation | 第69-71页 |
| 3.3.2 Confirmation of Transformation on Shoot induction medium | 第71-73页 |
| 3.3.3 Shoot elongation | 第73-76页 |
| 3.3.4 Root induction of the putative transformed shoots | 第76-78页 |
| 3.3.5 Confirmation of transgenic soybean | 第78-86页 |
| 3.4 Conclusion | 第86-87页 |
| Chapter 4. OPTIMIZATION OF MULTIPLE SHOOT INDUCTION OF JILIN SMALL 7GRANULE SOY | 第87-102页 |
| 4.1 Materials | 第87页 |
| 4.1.1 Plant materials | 第87页 |
| 4.1.2 Reagents, experimental equipments and instruments | 第87页 |
| 4.2 Methods | 第87-89页 |
| 4.2.1 Preparation of explants | 第87-88页 |
| 4.2.2 Media composition and preparation of cultures | 第88-89页 |
| 4.2.3 Statistical analysis | 第89页 |
| 4.3 Results and Discussion | 第89-101页 |
| 4.3.1 Germination rate of different sterilization times | 第89-90页 |
| 4.3.2 Effect BAP on in vitro multiple shoot formation | 第90-93页 |
| 4.3.3 Effects of different concentrations of Tria on TDZ (1 μM) on multiple shoot induction rate | 第93-95页 |
| 4.3.4 Effect of IBA and IAA on in vitro rooting medium | 第95-98页 |
| 4.3.5 Hardening of plantlets | 第98-101页 |
| 4.4 Conclusions | 第101-102页 |
| Chapter 5. CONCLUSION | 第102-104页 |
| REFERENCES | 第104-115页 |
| APPENDIX | 第115-120页 |
| VITA AND PUBLICATION | 第120-122页 |
| ACKNOWLEDGEMENTS | 第122-123页 |
