论文目录 | |
| ABSTRACT | 第1-8
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| TABLE OF CONTENTS | 第8-11
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| LIST OF FIGURES | 第11-13
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| LIST OF TABLES | 第13-14
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| CHAPTER | 第14-90
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| Ⅰ INTRODUCTION AND LITERATURE REVIEW | 第14-27
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| Rice as a model species for genome research | 第14-16
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| The large-insert DNA cloning systems and their utilities in genome research | 第16-20
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| Transforming plants with BIBACs or TACs carrying large DNA fragments | 第20-23
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| Genome-wide physical mapping | 第23-27
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| Ⅱ A PLANT-TRANSFORMATION-COMPETENT BIBAC-BASED INTEGRATED PHYSICAL MAP OF JAPONICA RICE FOR FUNCTIONAL ANALYSIS AND GENETIC ENGINEERING OF THE RICE GENOME | 第27-68
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| Abstract | 第27-28
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| Introduction | 第28-31
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| Materials and Methods | 第31-40
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| Source BIBAC and BAC libraries | 第31-35
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| Isolation and purification of BAC/BIBAC DNA | 第35-37
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| BAC and BIBAC fingerprinting and contig assembly | 第37-39
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| Library screening | 第39
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| BAC end sequencing | 第39
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| Availability of the integrated BAC/BIBAC-based physical map | 第39-40
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| Results and Discussion | 第40-68
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| BAC and BIBAC clones | 第40-42
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| BIBAC and BAC Fingerprinting | 第42-43
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| Contig Assembly and Manual Editing | 第43-51
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| Contig Reliability | 第51-57
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| BIBAC/BAC end sequencing and analysis | 第57-61
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| Utility of the BAC/BIBAC-based physical map for functional analysis of the rice genome sequence | 第61-62
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| Construction of a fine integrated genetic and physical map of rice chromosome 8 | 第62-68
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| Ⅲ CONSTRUCTION OF PLANT-TRANSFORMATION-COMPETENT BIBAC AND TAC LIBRARIES OF WILD AFRICA RICE (ORYZA BARTHII) FOR FUNCTIONAL ANALYSIS AND COMPARATIVE GENOMICS | 第68-90
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| Abstract | 第68-69
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| Introduction | 第69-71
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| Materials and Methods | 第71-78
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| Preparation of BIBAC and TAC vectors | 第71-73
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| Preparation of high-molecular-weight (HMW) DNA from nuclei | 第73-74
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| BIBAC and TAC Library construction | 第74-78
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| Analysis of BIBAC and TAC clones and library assembly | 第78
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| Results and Discussion | 第78-90
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| Construction of BIBAC library | 第78-79
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| Construction of TAC library | 第79-80
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| Analysis of BIBAC clones | 第80-84
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| Analysis of TAC clones | 第84-90
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| SUMMARY | 第90-92
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| REFERENCES | 第92-100
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| VITA | 第100-104
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| ACKNOWLEDGMENTS | 第104-105
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| APPENDIX (contigs of chromosome 8 of Oryza sativa L. ssp. japonica cv. Nipponbare) | 第105-115页 |
