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活血化瘀中药复方诱导K562细胞凋亡 |
[中药炮制论文]
作者:郑瑾,刘强,王宗仁,李军昌,王文,马爱玲,马静,李晶华,肖铁卉
【关键词】 中药复方 【Abstract】 AIM: To investigate the effects of blood-activating and stasis-eliminating compound on the proliferation and apoptosis of human chronic myelocytic leukemia cell line K562 cells. METHODS: Living cells were counted by trypan-blue excluding and cell growth curve was drawn. Changes of cell morphology were observed by light and transmission electron microscopy. Flow cytometry (FCM) was used to determine cell apoptosis quantitatively. RESULTS: Chinese herbal compound inhibited the proliferation of K562 cells. The apoptosis of K562 cells was confirmed by FCM and typical ultrastructural changes. The apoptosis rate of 100 ml・L-1 drug serum was higher than that of 50 ml・L-1 drug serum (13.4%±1.3% vs 7.6%±0.9%, P<0.01). CONCLUSION: Chinese herbal compound can inhibit the proliferation of human chronic myelocytic leukemia cell line K562 cells and induce the apoptosis of K562 cells. 【Keywords】 Chinese herbal compound; bloodactivating and stasiseliminating; apoptosis; K562 cell 【摘要】 目的: 研究活血化瘀复方对人慢性粒细胞白血病细胞株K562细胞增殖、凋亡的影响. 方法: 通过台盼蓝拒染法活细胞计数,绘制细胞生长曲线. 光镜、透射电镜观察细胞形态学变化. 流式细胞检测定量……
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中药炮制论文 | 中药炮制论文 |
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