[中药炮制论文]
【摘要】 高效液相色谱法测定复方养血生发片中何首乌有效成分二苯乙烯苷(2,3,5,4′四羟基二苯乙烯2OβD葡萄糖苷)的含量。方法 流动相为乙腈1%磷酸 (体积比15∶85);色谱柱为C18 柱;紫外检测波长为320 nm。结果 二苯乙烯苷在1.04~3.64 μg范围内具有良好的线性关系;平均回收率为99.36%,RSD为0.41%(n=9)。结论 该方法灵敏度、专属性高、重现性好,快速简便。 【关键词】 高效液相色谱法 二苯乙烯苷 复方养血生发片 Abstract:Objective To determinate the content of stilbene glycoside, an active ingredient of Polygonum multiflorum, in the compound Yangxueshengfa tablets by HPLC. Methods The mobile phase consisted of acetonitrile1% phosphoric acid (15∶85). The column was C18 column. A UVdetector was employed and the detection wavelength was 320 nm. Results 2,3,5,4’tetrahydroxystilbene2OβDglucoside has a good linearity within the concentration range of 1.04-3.64 μg/mL. The average recovery was 99.36% (n=9, RSD=0.41%). Conclusion The method is rapid, selective and reproducible. Key words:HPLC;stilbene glycoside; compound Yangxueshengfa tablets 复方养血生发片是由当归、地黄、五味子、制何首乌、熟地、木瓜等中药材制成的复方制剂。制何首乌具有补肝肾、益精血、乌须发的功效,主要含二苯乙烯苷(2,3,5,4′四羟基二苯乙烯2OβD葡萄糖苷)、大黄素、大黄酸等成分[1]。二苯乙烯苷为何首乌的特征成分,其含量测定方法有紫外分光光度法[2-3]、高效液相色谱法[4],但在药品标准中均没有收载。本文采用高效液相色谱法对其制剂中二苯乙烯苷含量进行测定,结果……
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