[生化制药论文]
作者:唐哲,文爱东,吴寅,赵磊,李宏力,石茹
【关键词】 卡马西平
Determination of carbamazepine concentration in human saliva by HPLC and its pharmacokinetic study
【Abstract】 AIM: To determine carbamazepine(CBZ) concentration in human saliva by HPLC and study its pharmacokinetics. METHODS: Shimpack CLCODS C18 (150 mm×6 mm, 5 μm) spectrum analytical column was used and methanolwater(53∶47)was adopted as the mobile phase. Flow rate was 1.0 mL・min-1, detection wavelength was 212 nm, column temperature was 23℃ and the dosage of CBZ was 50 μL. The pharmacokinetic parameters of CBZ tablets taken by 6 healthy volunteers at a single oral dose of 200 mg were calculated according to CBZ concentration in saliva. RESULTS: The standard curve was linear (r=0.999) within the range of 0.40~4.32 mg・L-1 for CBZ. The limit of detection in saliva was 30 μg・L-1 (S/N≥3). The average recovery was 99.17%-104.80%;the interday RSD and intraday RSD was 2.6%-4.9% and 3.0%-8.6% respectively.The results showed that the pharmacokinetics of CBZ was in accordance with the onecompartment open model. Tmax, Cmax, ke, T1/2 and AUC(0~inf) was (4.50±3.15) h, (1.52±0.22) mg・L-1, (0.0090±0.0006) h-1, (77.16±5.26) h and (132.18±8.82) mg ・h・L-1 respectively. After single dosing, the concentrationtime curve of CBZ in saliva presented multiple peaks. CONCLUSION: This method is simple, quic……
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