[生化制药论文]
作者:陈绍倩,杜英,黄玉敏,王鑫,顾巧丽,董子明
【摘要】 本 研究 探讨新型趋化因子CKLFSF8对人白血病HL60细胞的增殖和表皮生长因子受体(EGFR)表达的 影响 , 应用 RTPCR 方法 检测CKLFSF8基因在HL60细胞中的表达;利用脂质体将CKLFSF8基因转染到HL60细胞,在倒置显微镜下观察细胞生长;MTT法检测细胞的增殖程度;免疫细胞化学法检测CKLFSF8基因转染前后HL60细胞EGFR的表达。结果表明: 在细胞生长过程中可检测到CKLFSF8表达;CKLFSF8基因转染前后HL60细胞的增殖受到了明显的抑制,与空白对照组和空质粒组相比有显著性差异(P﹤0.05);对比转染前后细胞EGFR表达的阳性率具有显著性差异(P﹤0.01)。结论: CKLFSF8基因转染对白血病细胞HL60的增殖和EGFR的表达有明显抑制作用。
【关键词】 CKLFSF8; HL60细胞; 细胞增殖; EGFR
Effects of Novel Human Chemokinelike Factor Superfamily 8 on Proliferation and EGFR Expression of HL60 Cells
Abstract This study was aimed to investigate the effect of chemokinelike factor superfamily 8 (CKLFSF8) on proliferation and expression of epidermal growth factor receptor (EGFR) of HL60 cells. Expression of CKLFSF8 mRNA on HL60 cell line was assayed by RTPCR; the target gene was transfected into the cells by lipid vector, cell proliferation was determined by MTT assay, while expression of EGFR in HL60 was determined by immunocytochemical technique. The results indicated that expression of CKLFSF8 existed in HL60 &nb……
<<<<<全文未完,本文约2853个中文字,未计算英文字母、数字>>>>>
|
|