[生化制药论文]
作者:鲍文,陈宝安,高峰,丁家华,许文林1,沈惠玲1,高冲,孙耘玉,程坚,王骏,赵刚,马燕
【摘要】 本 研究 探讨环孢菌素A(CsA)、雌激素受体抑制剂雷洛昔芬及其联合 应用 对K562/A02细胞多药耐药的逆转作用。采用甲基四唑蓝法(MTT)测定柔红霉素(DNR)的半数抑制量,RT-PCR法检测mdr1基因mRNA表达水平,FCM检测P-gp的表达和细胞内DNR浓度。结果表明: DNR对K562/A02和K562细胞的IC50分别为23.51和0.29mg/L,雷洛昔芬(2.5 mg/L)及CsA( 1 mg/L)单用和两药联合处理K562/A02细胞时, DNR的IC50值分别为5.98、8.15和3.68 mg/L,两药对DNR作用K562细胞的IC50没有 影响 。CsA及雷洛昔芬单独作用后耐药株mdr1 mRNA下调微弱,联合用药下调效果明显。CsA及雷洛昔芬均可降低P-gp蛋白的表达,且具有协同作用。同时还观察到逆转剂雷洛昔芬和CsA作用后细胞内柔红霉素浓度增加,两药联合作用时效果增强。结论: CsA及雷洛昔芬均可逆转耐药,且联合作用效果增强。
【关键词】 环孢菌素A; 雌激素受体抑制剂; 雷洛昔芬; K562/A02细胞; 多药耐药
Effect of Cyclosporine A, Raloxifene and The ir Combination on the Reversion of Multidrug Resistace of K562/A02 Line
AbstractThis study was aimed to investigate the reversible effect of cyclosporine A, raloxifene and their combination on multidrug resistance cell line K562/A02. The IC50 (the concentration causing 50% inhibition of cell growth) of DNR were assayed by MTT method, the expression level of mdr-1 mRNA was assayed by RT-PCR, p-glycoprotein (P-gp) expression and intracellular DNR concentration were detected by flow cytometry. The results showed that the IC50……
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