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流式微珠阵列法检测IFN-γ诱导的Jurkat细胞Th1/Th2细胞因子表达药学 |
| [生化制药论文]
【摘要】 本 研究 旨在探讨流式微珠阵列法检测Th1/Th2细胞因子表达及IFN-γ 应用 于T淋巴细胞白血病 治疗 的临床价值。以不同浓度IFN-γ诱导培养Jurkat细胞48小时，用流式微珠阵列法检测培养上清液中IL-2、IL-6、TNF-α和IFN-γ表达，并采用流式细胞术检测膜上IL-2受体（CD25）的表达。结果表明： IFN-γ诱导Jurkat细胞IL-2和TNF-α的表达呈浓度依赖性升高，未检测到IL-6表达，CD25表达明显升高。结论： Jurkat细胞能被IFN-γ诱导高表达Th1细胞因子和CD25，流式微珠阵列法能准确客观地反映Th1/Th2细胞因子表达的动态变化。
【关键词】 T淋巴细胞白血病
Detection of Expression of Th1/Th2 Cytokines in Jurkat cell Treated with γ-Interferon by Cytometric Bead Array
Abstract In order to explore the value of γ-interferon (IFN-γ) in therapy of T lymphoid leukemia and role of cytometric bead array (CBA) method in detection of Th1/Th2 cytokines expression，the Jurkat cells were cultured for 48 hours in different concentration of IFN-γ，the expressions of IL-2，IL-6，INF-γ and TNF-α in culture supernatants were assayed by CBA method; CD25 expression was assayed by flow cytometry. The results showed that the expressions of IL-2，TNF-α were enhanced in INF-γ dose-dependent way，and the expression of CD25 was also enhanced，but there was no expression of IL-6. It is concluded that Jurkat cells induced by IFN-γ were able to express high-level of Th1 cytokine and IL-2 membrane receptor (CD25)，and the CBA method can be used to exactl……
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| 投稿人：7uy |
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最后编辑：fibg |
生化制药论文 | 生化制药论文 |
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