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TEM116型ESBL天然酶与重组酶的动力学特性研究药学 |
| [生化制药论文]
作者：曾贤铭 陈秀枢 王震 吕建新 楼永良 孙长贵 陆永绥
【摘要】 目的 研究 TEM116型ESBL天然酶与重组酶的动力学特性，并比较它们的差异。 方法 采用紫外分光光度法检测酶促抗生素水解反应，以LeeWilson改良双倒数方程数据处理法进行数据处理，测定天然酶与重组酶的Km、Vmax及kcat。观察温度和pH对酶促反应的 影响 。结果 以LeeWilson改良双倒数方程数据处理法进行数据处理方便而准确地测定了天然酶与重组酶的Km与Vmax并 计算 出kcat。温度和pH对天然酶与重组酶酶促反应效应相似。天然酶与重组酶最优先的底物均为头孢哌酮，其次是头孢氨苄；对氨苄西林、阿莫西林、青霉素和哌拉西林有最高的催化效率。结论 天然酶与重组酶的动力学参数无显著差异。
【关键词】 动力学 β内酰胺酶 重组蛋白 β内酰胺类抗生素
Kinetic characteristics of native and recombinant TEM116 betalactamases
ABSTRACT Objective To investigate and compare the kinetic characteristics of native and recombinant TEM116 betalactamases. Methods Hydrolysis reactions of betalactam antibiotics were detected by ultraviolet photometry. Data was processed according to Lee and Wilson′s improved doublereciprocal equation. Kinetic parameters of native and recombinant TEM116 betalactamases, including Km, Vmax and kcat, were determined. Effects of temperature and pH on enzymatic reaction were observed. Results Michaeles constant (Km) and maximal velocity (Vmax) were obtained by means of Lee and Wilson′s improved doublereciprocal equation, and the catalyti……
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| 投稿人：7uy |
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最后编辑：fibg |
生化制药论文 | 生化制药论文 |
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