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高效液相色谱法测定康妇灵胶囊中苦参碱、氧化苦参碱的含量药学 |
[生化制药论文]
【摘要】 目的:建立康妇灵胶囊的含量测定 方法 。方法:采用高效液相色谱法。色谱柱:Agilent Technologies ZORBAX Extend-C18 4.6×250mm,5μm,流动相:乙腈-甲醇-磷酸盐缓冲液(PH6.8)(16: 16:68),流速:1.0ml·min-1,检测波长:220nm,柱温:30℃。结果:苦参碱在0.02~0.40mg·ml-1范围内线性关系良好(r=0.9995);平均加样回收率为98.2%,RSD=1.4%(n=6);氧化苦参碱在0.01~0.20mg·ml-1范围内线性关系良好(r=0.9997);平均加样回收率为97.6%,RSD=2.2%(n=6)。结论:该方法简便易行,结果准确可靠,可适用于康妇灵胶囊的质量控制。
【关键词】 高效液相色谱法 康妇灵胶囊 苦参碱 氧化苦参碱 含量测定
Determination of Matrine Oxgmatrine content in Kangfuling capsules by HPLC
【Abstract objective】To set up a method for quality control of Fuyankang tablets. METHOD:HPLC method was developed to quantitative determination. The separation was performed on Agilent Technologies ZORBAX Extend-C18 4.6×250mm,5μm.The mobile phase was acetonitrile-methanol-phosphate buffer (pH=6.8) (16:16:68).The flow rate was 1.0ml·min-1 .The UV detection wavelength was 220nm. The column temperature was 30℃.RESULTS:The linear range of Matrine and Oxgmatrine were at 0.02~0.40 mg·ml-1 (r=0.9995) and 0.01~0.20 mg·ml-1 (r=0.9997) respectively, the average recoveries(n=6) were 98.2% and 97.6% with RSD 1.4% and 2.2%. CONCLUSION The method is simple and accurate, it can be used for quality control of Kangfuling capsules.
【Key words】HPLC; Kangfuling capsules;Matrine;Oxgmatrine; Determination
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投稿人:7uy |
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最后编辑:fibg |
生化制药论文 | 生化制药论文 |
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