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高效液相色谱法测定康妇灵胶囊中苦参碱、氧化苦参碱的含量药学 |
| [生化制药论文]
【摘要】 目的：建立康妇灵胶囊的含量测定 方法 。方法：采用高效液相色谱法。色谱柱：Agilent Technologies ZORBAX Extend-C18 4.6×250mm，5μm，流动相：乙腈-甲醇-磷酸盐缓冲液（PH6.8）（16: 16:68），流速：1.0ml·min－1，检测波长：220nm，柱温：30℃。结果：苦参碱在0.02～0.40mg·ml－1范围内线性关系良好（r=0.9995）；平均加样回收率为98.2%，RSD=1.4%（n=6）；氧化苦参碱在0.01～0.20mg·ml－1范围内线性关系良好（r=0.9997）；平均加样回收率为97.6%，RSD=2.2%（n=6）。结论：该方法简便易行，结果准确可靠，可适用于康妇灵胶囊的质量控制。
【关键词】 高效液相色谱法 康妇灵胶囊 苦参碱 氧化苦参碱 含量测定
Determination of Matrine Oxgmatrine content in Kangfuling capsules by HPLC
【Abstract objective】To set up a method for quality control of Fuyankang tablets. METHOD：HPLC method was developed to quantitative determination. The separation was performed on Agilent Technologies ZORBAX Extend-C18 4.6×250mm,5μm.The mobile phase was acetonitrile-methanol-phosphate buffer (pH=6.8) (16：16：68).The flow rate was 1.0ml·min-1 .The UV detection wavelength was 220nm. The column temperature was 30℃.RESULTS：The linear range of Matrine and Oxgmatrine were at 0.02～0.40 mg·ml-1 (r=0.9995) and 0.01～0.20 mg·ml-1 (r=0.9997) respectively, the average recoveries(n=6) were 98.2% and 97.6% with RSD 1.4% and 2.2%. CONCLUSION The method is simple and accurate, it can be used for quality control of Kangfuling capsules.
【Key words】HPLC； Kangfuling capsules；Matrine；Oxgmatrine； Determination
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| 投稿人：7uy |
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最后编辑：fibg |
生化制药论文 | 生化制药论文 |
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