【关键词】  银杏叶提取物；,,血红素氧合酶-1；,,再灌注损伤；,,凋亡

　　摘要：目的探讨银杏叶提取物（EGb761）诱导血红素氧合酶-1（HO-1）表达抗缺血再灌注损伤（IRI）后心肌细胞凋亡的效果及其机理。方法48只兔随机均分为单纯IRI组、EGb761组、EGb761+ZnPPⅨ组，分别预先给予等容积的生理盐水，EGb761，EGb761+ZnPPⅨ腹腔内注入。24 h后，每组任选8只处死，检测心肌细胞内HO-1表达及活性，余兔结扎冠脉左前降支使心肌缺血40 min，再灌注4 h，取缺血心肌检测中性粒细胞浸润、丙二醛（MDA）含量、Bax蛋白与Bcl-2蛋白表达及心肌细胞凋亡指数（AI）。结果与单纯IRI组及EGb761+ZnPPⅨ组比较，EGb761组HO-1表达及活性均明显升高，中性粒细胞浸润及MDA含量明显降低，Bax 增加而Bcl-2降低，心肌细胞AI显著减少(均为P<0.01)。与单纯IRI组比较，EGb761+ZnPPⅨ组HO-1表达增加但活性降低(P<0.05)，MDA含量明显升高(P<0.05)，其余指标均无显著差异。结论EGb761能明显诱导心肌细胞内HO-1表达，并通过HO-1的抗炎、抗氧化等作用下调Bax表达，上调Bcl-2表达，从而显著抑制IR损伤后心肌细胞凋亡。

　　关键词：银杏叶提取物；  血红素氧合酶-1；  再灌注损伤；  凋亡

　　Abstract：ObjectiveTo explore the protective effects and mechanism of heme oxygenase-1 (HO-1) expression induced by extract of Ginkgo biloba(EGb761) against cardiomyocyte apoptosis from ischemia reperfusion injury(IRI).Methods48 rabbits were randomly divided into simple IR group, EGb761 group and EGb761+ ZnPPⅨ group. Each rabbit in different groups was intraperitoneal injected with saline(5ml), EGb761(10mg/kg), EGb761(10mg/kg)+ ZnPPⅨ(7.5mg/kg) respectively. 24 hours later, 8 rabbits in each group were killed to measure myocardial H……