[生化制药论文] 【摘要】 目的 建立病毒消口服液中绿原酸含量的高效液相色谱法。方法 色谱柱为Hypersil ODS C18(5μm,4.6mm×100mm),流动相为乙腈-0.4%磷酸溶液(13∶87),检测波长为327nm;柱温为25℃;流速为1.01ml/min。结果 绿原酸在112~1120ng(r=0.99998,n=6)范围内呈良好的线性关系;平均回收率为99.08%,RSD为0.57%。结论 本方法简便、快速,测定结果准确,重现性好。 【关键词】 病毒消口服液;绿原酸;高效液相色谱法;含量 Determination of the contents of chloralgenic acid in Bingduxiao Koufuye by HPLC
【Abstract】 Objective To establish a HPLC method for determining the contents of chloralgenic acid in Bingduxiao Koufuye.Methods Stationary phase was Hypersil ODS C18(4.6min×100mm;5μm),mobile phase was acetonitrile-0.4% H3PO4 triethylamine(13:87),election wavelength 327nm,flow rate 1.0ml/min and coulumn temperature 25℃.Results There was good linearity in range of 112~1120ng(r=0.99998,n=6) for chloralgenic acid.The average recovery of chloralgenic acid was 99.08%;RSD was 0.57%.Conclusion This method is simple,fast and accurate.
【Key words】 Bingduxiao Koufuye;chloralgenic acid;HPLC;content
病毒消口服液为我院内科传统制剂,具有清热解毒,祛湿,凉血作用,用于治疗上呼吸道感染、腮腺炎、风热感冒,瘟热等病毒性疾患。该制剂由银花、板蓝根、连翘、重楼等组成。为更好地控制该制剂质量,保证用药安全有效,笔者进行了定量研究,采用高效液相色谱法测定方中绿原酸的含量,现报告如下。
1 仪器与试药
<……
<<<<<全文未完,本文约1354个中文字,未计算英文字母、数字>>>>>
|