| [高血压医学论文] ［摘 要］ 目的：探讨核酸扩增(PCR)微板杂交捕获检测宫颈脱落细胞内高危型人乳头状病毒(HPV)感染的临床意义，建立快速、灵敏的检测方法。方法：采用PCR微板杂交捕获检测140例宫颈脱落细胞高危型HPV(HPV16/18/31/33/35/39/45/51/52/56/58/59/68)感染状况。结果：细胞学诊断低度鳞状上皮内病变(LSIL)56例，高危型HPV DNA阳性18例(阳性率为32.5%)；细胞学诊断高度鳞状上皮内病变(HSIL)42例，高危型HPV DNA阳性25例(阳性率为59.5%)；未明确诊断意义的不典型鳞状上皮细胞(ASCUS)31例，高危型HPV DNA阳性11例(阳性率为35.5%)；鳞状细胞癌9例，高危型HPV阳性8例(阳性率88.9%)。结论：PCR微板杂交捕获检测宫颈脱落细胞内高危型HPV感染，具有快速、简便、灵敏度高、特异性强等优点，适用于大规模筛查和临床常规工作。
［关键词］ 宫颈脱落细胞；人乳头状病毒；微板杂交捕获法
The Clinic Meaning of Cervical Cytological Speciments HPV Microplate Hybrid Capture Detection PCR Assays
Abstract： Objective To establish a rapid and sensitive method of detect cervical hiskrisk human papillomavirus infections in cervical cytological speciments. Methods HPV type 16/31/33/35/39/45/51/52/56/58/59/68 DNA were detected in 140 cervical specimens by Microplate Hybrid Capture Detection PCR Assys. Results In cervical cytological speciments of 56 LSIL ,18cases were positive highrisk HPV DNA(32.5%);25 cases were positive highrisk HPV DNA in 42 HSIL cytological speciments(59.5%);Among 31 ASCUS specimens,11 cases were positive highrisk HPV DNA(35.5%);for the other 9 cervical squmaous cell carcinomas specimens, 8 cases were positive highrisk HPV DNA(88.9%).Conclusion Microplate H……
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