|
|
实时荧光PCR和RT-PCR方法检测登革病毒的比较研究 |
| [高血压医学论文] 作者：刘建军，古丽巴哈尔，阳 帆，陈家敏，何雅青，杨 洪
【摘要】 目的 通过2种检测登革病毒方法的比较，以提高检测登革病毒的灵敏度和特异性。方法 利用Taqman MGB技术，根据登革病毒3′端非编码区的一段高度保守序列，设计登革1～4型荧光PCR通用引物和TaqmanMGB探针，以登革热毒株作为标准，以乙脑毒株作对照，建立实时荧光PCR检测登革病毒的快速方法。并对10份ELISA法检测阳性的临床血清标本进行RT-PCR及荧光PCR扩增。结果 RT-PCR检测10份临床血清标本，2份阳性，阳性率为20%。实时荧光PCR检测登革病毒与乙脑病毒无交叉反应，检测10份临床血清标本，5份阳性，阳性率为50％。从RNA提取到检测结果仅需4h。结论 Taqman MGB实时PCR检测方法快速、敏感性高、特异性强，可作为登革病毒的快速检测方法，应用于登革热的临床早期诊断。 【关键词】 登革病毒；RT-PCR；Taqman GMB实时PCR 【Abstract】 Objective In order to improve the sensitivity and specificity of detecting dengue viruses， two methods for detecting dengue viruses were compared.Methods Using Taqman MGB technique， a pair of universal primers and Taqman MGB probe were designed according to a highly reserved sequence of the 3′-noncoding region of dengue viruses type 1-4. Dengue virus strains were used as standard and Japanese encephalitis virus strains were used as control， the real-time PCR assay for specific and sensitive detection of the dengue viruses was established. While 10 serum specimens of ELISA positive were detected by the RT-PCR and fluorescent PCR.R……
<<<<<全文未完，本文约3087个中文字，未计算英文字母、数字>>>>>
|
|
| |
| 投稿人：gf6h6 |
|
最后编辑：647754 |
高血压医学论文 | 高血压医学论文 |
|
