【关键词】  鬼针草；,,总黄酮；,,大孔树脂

　　摘要：目的 研究 大孔吸附树脂富集纯化鬼针草总黄酮的最佳工艺条件及参数。 方法 采用不同浓度的乙醇梯度洗脱,以紫外分光光度法测定鬼针草总黄酮收率和纯度为指标。结果实验条件下，采用D301型大孔树脂进行吸附,20%乙醇100 ml洗脱，经大孔树脂处理后浸膏的总黄酮含量可达60%以上。结论 该法可为鬼针草新制剂的研制与生产提供原料药。

　　关键词：鬼针草；  总黄酮；  大孔树脂

　　Study on the Isolation and Purification of Total Flavonoids from Bidens bipinnataL. with Macroporous Resin

　　Abstract：ObjectiveTo explore the optimal purification condition of total flavonoids from Bidens bipinnata L. with macroporous resion.MethodsGradient elution was used to elute the components absorpted on macroporous resin guided by the isolation-purification degree and the recovery rate.Results The content of total flavonoids in obtained ointment could reach 60% under the condition that D301 type macroporous resin was used as absorption agent and 100 ml 20% ethanol as elution agent.ConclusionThe method appeared to be feasible to isolate and purify total flavonoids from Bidens bipinnata L..

　　Key words：Bidens bipinnata L.;   Total flavonoids;  Macroporous resin
   
　　鬼针草为菊科植物鬼针草的全草，具清热解毒、散瘀消肿之功效［1］，药理和临床报道对原发性高血压有明显疗效，其降压有效成分主要为黄酮类化合物［2］。 目前 国内上市的有关鬼针草提取物的制剂主要有鬼针草颗粒剂、悦年片、悦年茶等，鬼针草药源充足，价格低廉，开发前景广阔。本实验采用大孔树脂……