[高血压医学论文] 【关键词】 国产试剂盒;HBV
[摘 要] 目的:探讨上海复星、深圳匹基、上海科华三种国产乙型肝炎病毒基因(HBVDNA)荧光定量试剂的临床检测结果相互之间是否存在差异。方法:将相同的标本分别用三种国产HBVDNA荧光定量试剂进行HBVDNA荧光定量测定。然后再对检测结果进行对比,分析相互之间是否存在差异。结果:23例已知的慢性乙型肝炎患者标本,3例已知正常标本分别用三种国产HBVDNA荧光定量试剂进行HBVDNA定量检测,结果显示三种国产试剂的HBVDNA定量检测结果相互之间差异小于一个数量级占91.4%,大于一个数量级小于二个数量级的占8.6%,经统计学分析差异无显著意义(P>0.05)。结论:上述三种国产HBVDNA荧光定量试剂在同一套设备上的检测结果相互之间差异很小,有很好的准确度,它们的检测结果可以相互进行比较。
[关键词] 国产试剂盒;HBVDNA荧光定量PCR;差异
Comparison Study Among the 3 Domestic Fluorescence Quantitative PCR Diagnostic Kits for HBVDNA
Abstract:Objective To study the difference of the commmerical fluorescence quantitative PCR diagnostic kits for HBVDNA,which were made in china.Methods 26 samples.which we collected from our clinical lab,23 of its were positive in HBVDNA and 3 of its were negative.which were detected by the 3 fluorescence quantitative PCR diagnostic kits.Results There was a good correlation among the 3 domestic fluorescence quantitative PCR diagnostic kits for HBVDNA.No significant difference were observed among the 3 methods.Conclusions There were no significant difference among the 3 domestic diagnostic kits,which were fit for the routine use in the clinical lab,the results can be compared each other.……
<<<<<全文未完,本文约2044个中文字,未计算英文字母、数字>>>>>
|