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心脏缺血再灌注损伤后一氧化氮合酶的变化 |
[护理学论文] 作者:唐省三,马亚珍,刘红云,朱晓琴,雷水生 【关键词】 再灌注损伤 Changes of nitric oxide synthesis in cardiac ischemia reperfusion injury 【Abstract】 AIM: To investigate the expression and function of endothelial nitric oxide synthase (eNOS) and inducible NOS (iNOS) during ischemiareperfusion injury (IRI) in rat hearts. METHODS: IRI was induced by clamping the left anterior descending (LAD) of coronary artery for 1h, followed by reperfusion. Nitric oxide synthase (NOS) activities of hearts was assayed at various time points and NOS protein levels as well as NOS mRNA expression were determined by Western blot and RTPCR methods respectively. RESULTS: eNOS activity, protein level and mRNA expression all increased after 26 h of IRI and decreased after 3 d of IRI and then gradually returned to basal level after 21 d. iNOS was expressed after 12 h of IRI and reached the peak level at day 3 after IRI and then decreased to basal level. CONCLUSION: The expression of iNOS is high while that of eNOS is low during cardiac ischemiareperfusion injury. Proper regulation of eNOS and iNOS expression may be therapeutically helpful in treating patients with cardiac ischemiareperfusion injury. 【Keywords】 reperfusion injury; nitric oxide synthase; heart 【摘要】 目的: 研究大鼠心脏缺血再灌注损伤(IRI)过程中一氧化氮合酶(NOS) 的变化规律及其作用. 方法: 制作SD大鼠心脏IRI动物模型,用同位素法测定正常及IRI心组织的NOS活性;用Western印迹和逆转录PCR法分析eNOS和iNOS 在IRI过程中蛋白和基因表达的变化……
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护理学论文 | 护理学论文 |
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