[中等职业教育论文] 摘 要：漆酶最早是从漆树所产的生漆中发现的，广泛分布于高等植物和真菌，在昆虫和细菌中也有发现。它是一种含铜的多酚氧化酶，既是聚合酶，也是降解酶，参与愈伤组织形成、木质化过程、生漆成膜、氧化酚类、苯胺类等一系列有机化合物。漆酶的去甲基化作用可以使木质素降解，也能够参与自然界中花色的形成。漆酶的应用以分离纯化为基础，主要用到沉淀、透析、色谱、电泳等技术。
关键词：漆酶; 来源; 分离纯化;
Abstract: Laccase, firstly found in the raw lacquer of Rhus vernicifera , is widely distributed in higher plants and fungi, and has been found also in insects and bacteria. As polyphenol-oxidase containing copper, it is polymerase as well as lyase that joins renewal of tissue, lignifications, formation of lacquer film, oxidation of a broad range of organic substrates, including phenols, anilines, etc. The demethylation of laccase is applied to lignin degradation and formation of color within natural flowers. Isolation and purification is the foundation of application, including sedimentation, dialysis, chromatograph and electrophoresis mainly.
Keywords: Laccase; Origin; Isolation and purification;
漆酶(Laccases，Lac， EC1.10.3.2)，即对苯二酚: 氧氧化还原酶，又名酚酶，多酚氧化酶等，是一种含铜的糖蛋白氧化酶，它和植物中的抗坏血酸氧化酶、哺乳动物的血浆铜蓝蛋白同属蓝色多铜氧化酶(blue multi-copper oxidase)家族[1]。在生漆中，漆酶是生漆固化成膜的生物催化剂，它催化漆酚形成聚合物。在过去的数十年，漆酶由于其广泛而绿色的可应用价值，备受研究者和生产加工者的关注，其身影几乎出现在化学……
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